Array Tape platform0 pages

Douglas

SCIENTIFIC

Array Tape™ Platform provides HTP Screening

with Reduced Plastic, Reagent and Energy Consumption

Mike Salentine and Kjersten Larson-Cook, Ph.D.

Overview

Purpose - Compare environmental impact of Array Tape Platform vs.

microplate-based technology for high throughput (HTP) processing.

Methods - Plastic consumption, reaction volumes, overall laboratory

footprint and energy use were compared in labs processing 200,400 or

600 384-well arrays per day.

Results - All parameters at all throughputs were reduced using the

Array Tape platform compared to microplate-based technology.

Conclusions - Array Tape and customized Platform provide

environmental benefits to HTP laboratories based on reduced plastic

consumption, reaction volumes, laboratory footprint and energy use.

Introduction

The Array Tape Platform is an automated, HTP technology based on

a continuous strip of a light and flexible polymer that is serially

embossed with reaction wells in customized volumes and formats.

Inline, parallel automation provided by the Array Tape liquid

handling (Nexar) and detection (Araya) instruments speeds processing

and reduces wasted motion inherent to robotically driven

microplate-based processes.

The Array Tape Platform has proven to increase throughput,

flexibility and cost savings in HTP laboratories that previously used

robotically driven, microplate-based technology.

We will demonstrate how the properties of Array Tape and

customized Platform also provide environmental benefits based on a

comparison of plastic consumption, reaction volumes, laboratory

footprint and energy efficiency.

Reducing Plastic Consumption

Plastic consumption is based on the amount of material required to

manufacture an SBS array of reaction wells in Array Tape vs. a

commonly used 384-well PCR microplate (Axygen Biosciences, RGD-C).

Plastic Consumption

■ Array Tape ■ Microplate

Number of Plates Processed

Results

• ~7 times more plastic is required to manufacture a single microplate

(21.3 g) compared to a 384-well equivalent in Array Tape (3.1 g).

• Array Tape reduces plastic consumption by as much as 11,000 g per

day (~25 lbs) in HTP laboratories processing 600 Arrays per day.

Miniaturizing Reaction Volumes

Array Tape is optimized for small volume reactions. Standard reaction

volumes in Array Tape were compared to 384-well microplates.

Results

• Array Tape reduces minimum

practical reagent volume to < 800

nL per well compared to 5 ul in

microplates.

• Array Tape minimizes the need to

store, handle and dispose of

potentially hazardous chemicals.

Reducing Laboratory Footprint

Compact Storage

Array Tape is thin and flexible - 200 microplate equivalents (76,800 reaction

wells) spooled onto a single, compact reel (90 mm by 560 mm).

Results

• Storage requirements for 200

SBS arrays (384-well) in Array

Tape vs. microplates

Inline Automation

Indexing holes running along each edge of the Array Tape guide reaction

wells through liquid handling (Nexar) and scanning (Araya) instruments.

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Results

• Array Tape Platform reduces laboratory footprint by minimizing robotics

and manual handling required to move microplates from station to station.

Reducing Laboratory Footprint & Energy Use

Thermocycling in Soellex

A comparison of the instrumentation (laboratory footprint) and energy

use (kWh) required to thermocycle samples in Array Tape (Soellex) vs.

microplates (water baths or block heaters).

Soellex

The heating system in the

water bath tightly controls the

thermal gradients throughout

the water column and provides

rapid energy transfer from the

water through the Array Tape

reaction welis.

• Soellex: 3-chambered ultra-HTP PCR thermal cycler optimized

for Array Tape

• Simultaneously thermocycles up to 3 spools of Array Tape

• 230,400 reaction wells thermocycled in a single process run

Energy Use

200 400 600

Number of 384-well Arrays Processed

Results - 600 Arrays per Day

• Array Tape (3 spool capacity): A single Soellex (26.4 kWh)

• Water Bath (32 microplate capacity): 18 water baths and ~11 times

more power (277.2 kWh)

• Block heater (4 microplate capacity): 150 heaters and ~35 times

more power (940.8 kWh)

Conclusion

The Array Tape Platform provides an environmentally responsible

solution for laboratories striving to achieve HTP processing with

minimal impact to the environment.