TemPro - Stand-alone Benchtop, Filter-based Spectrofluorometer0 pages

ELEMENTAL ANALYSIS
FLUORESCENCE
FLUORESCENCE
GRATINGS &
OEM SPECTROMETERS
OPTICAL COMPONENTS

PARTICLE CHARACTERIZATION
RAMAN
SPECTROSCOPIC ELLIPSOMETRY
SPR IMAGING

TemPro
Fluorescence lifetime system

Affordable fluorescence lifetime spectroscopy for every laboratory
TemPro lifetime spectrofluorometers, unique in their sensitivity and selectivity, allow researchers to record fluorescence lifetimes,
offering a dynamic picture of a sample’s characteristics, including molecular motion and size, solvation environment, and
intermolecular distances—information that steady-state fluorescence cannot provide. They also offer the ability to temporally
separate similar fluorescence spectra from two distinct species in a solution.
TemPro uses Time-Correlated Single-Photon Counting (TCSPC)—the reliable, intuitive, and most sensitive lifetime-measurement
technique. The filter-based TemPro can measure a complete range of lifetimes from as short as 100 picoseconds to 1 second, with our
NanoLED and SpectraLED solid-state pulsed excitation sources spanning wavelengths from the UV to NIR.

Applications
Stern-Volmer quenching
FRET (Förster Resonance
Energy Transfer)
Efficiency of photosynthesis
Deconvolution of mixing processes
Fluorescence anisotropy
Fluorescence decay of 10–5 M aqueous
Erythrosin B, taken with the TemPro,
giving a lifetime of 85 ps. Excitation
= 488 nm; emission > 550 nm. The
green line is the mono-exponential
fit to the decay data (red dots) after
reconvolution with the instrumental
response (blue dots).

Anisotropy decay of 10 M DPH in
Kaydol®, recorded with the TemPro.
Excitation = 373 nm; emission > 418
nm. The initial anisotropy of 0.33 drops
to zero showing a free rotation with
4.9 ns correlation time.
–5

Protein denaturation