Reproducibility Studies NanoDrop 2000/2000c0 pages

PERFORMANCE DATA 

    NanoDrop 2000/2000c

Reproducibility Studies
Introduction
The precision or reproducibility of the Thermo Scientific NanoDrop 2000 Spectrophotometer was assessed measuring 10
individual aliquots of 8 concentrations of herring sperm dsDNA. Between each measurement, the sample was removed with
a dry, lint free lab wipe and a fresh aliquot of dsDNA was analyzed.
A second reproducibility study on the NanoDrop™ 2000c was conducted using 8 different concentrations of herring sperm
DNA diluted in azide buffer. Following a blank measurement with the buffer, 5 separate aliquots of the DNA sample
solutions were measured using a 1 cm quartz cuvette.
NanoDrop 2000/2000c pedestal
Measurement 1
Measurement 2
Measurement 3
Measurement 4
Measurement 5
Measurement 6
Measurement 7
Measurement 8
Measurement 9
Measurement 10
Average (ng/uL)
St Dev (ng/uL)
% CV

5.4
5.0
5.3
5.4
5.9
5.5
4.0
5.6
5.2
6.0
5.3
0.6
N/A

28.9
28.8
28.8
29.3
28.5
29.5
29.5
29.7
29.9
29.6
29.3
0.5
N/A

57.8
58.7
58.0
57.9
60.0
58.0
58.1
58.6
59.0
58.2
58.4
0.7
N/A

290.5
296.3
294.0
293.5
293.7
295.3
292.7
292.3
294.1
293.5
293.6
1.6
0.54

570.5
577.7
569.9
569.2
568.3
567.2
568.1
567.7
574.4
567.5
570.1
3.4
0.60

1713.6
1705.5
1735.0
1715.0
1721.2
1708.9
1732.8
1712.9
1743.2
1720.0
1720.8
12.4
0.72

3553.6
3587.2
3563.3
3587.0
3583.0
3551.6
3574.4
3539.7
3561.7
3555.1
3565.7
16.5
0.46

11377.2
11215.5
11272.7
11312.3
11280.0
11245.9
11337.3
11302.7
11246.4
11402.1
11299.2
59.6
0.53

0.4
0.4
0.3
0.3
0.2
0.3
0.08

0.6
0.7
0.6
0.5
0.5
0.6
0.08

0.9
0.9
1
1
1
1.0
0.05

2.2
2.2
2.1
2.2
2.3
2.2
0.07

4.1
4.1
4.1
4.1
4.1
4.1
0

24.7
24.6
24.8
24.8
24.8
24.7
0.09

48.6
48.6
48.7
48.8
48.8
48.7
0.1

71.9
72.3
72.1
71.8
72.3
72.1
0.23

Reproducibility
2 - 100 ng/uL SD: ±2ng/uL
>100 ng/uL to ~15000 ng/uL %CV: 2%

NanoDrop 2000c cuvette (1cm quartz )
Measurement 1
Measurement 2
Measurement 3
Measurement 4
Measurement 5
Average (ng/uL)
St Dev (ng/uL)
Reproducibility
0.4 ng/uL to 10 ng/uL SD: ±0.15 ng/uL
Rev 3/09
Thermo Fisher Scientific - NanoDrop products

Wilmington, Delaware USA

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