Tempoâ„¢ ht LC System0 pages

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Technical Note

nnHigh Performance Nanoflow Chromatography with the Tempo nano LC Systems nn

Making reliable low flow separations a reality!

Christie L. Hunter >
1 1 , and David M. Cox >
2 2 Applied Biosystems, USA, MDS Sciex,Canada From simple protein identification experiments to global protein expression workflows, proteomic applications require sensitive, reproducible, reliable chromatography. Tempo nano LC systems set a new performance standard for low flow separations. >

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Technology Overview

The Tempo nano LC systems employ a direct pumping system with flow meters monitoring the flow rate in each mobile phase to a precision of <1 nL/min. Flow rate information is continuously fed to a microfluidic flow controller which rapidly adjusts a variable pressure source. This allows the system to maintain precise flow rates from 20-1000 nL/min. Each mobile phase is monitored independently before mixing, providing for precise gradient formation (Figure 1). The simplicity in design of the Tempo nano LC systems represents a significant improvement in ease-of-use and reliability for the proteomics researcher. This direct pumping system eliminates the need for flow splitting, alleviating the flow inaccuracies of splitter based systems and reducing solvent consumption and waste. Close coupling of the pump, gradient mixer and autosampler minimizes dispersion and delay time. Flow rate is unaffected by downstream pressure fluctuations, increasing the long term run to run stability of these low flow separations. >

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Key Advantages of the Tempoٙ nano LC Systems

Օ Direct pumping system and the measurement of flows with active feedback ensures precise gradient delivery, providing excellent run-to-run reproducibility Optimized for low flow rates between 201000 nL/minute, (and 1-20 ֵ L/min for the first dimension pump in MDLC system) Օ Ultra low delay volume of 65 nL from mixer to injection valve enables rapid gradients and re-equilibration to shorten total cycle times Elimination of flow splitting reduces operating costs due to low solvent consumption and minimal waste production Full MDLC functionality available Օ Easy-to-use software interface Simplified plumbing scheme and split-less design make for a reproducible, easy-to-use system >
Figure 1. Real-time monitoring of flow ratesfor each mobile phase at 500 nL/min. Microfluidic flow control provides a high level of flow accuracy for each channel, enabling very precise gradient formation. www.appliedbiosystems.com >

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